Pattern Genetics - Non-linear Cell Dynamics

Signal Transduction Network and Gene Regulated Expression Involved in Neuro-Endocrine Cytogenesis
神经内分泌细胞发生的信号传导网络和基因表达调控
Ben J. Zeng

PART I. Introduction and Purpose: Self-organization of Genomic Biosystems

A) Cell, molecular and pattern genetics
1). Cytogenetics - medical genetics
2). Molecular biology of gene
3). Pattern (system - structure) genetics, from gene to genome
4). Genomics and pan-evolution
B) Neuroendocrine pathways of pituitary hormone regulation
1). Ontogeny of somatotroph, gonadotroph and pituitary hormones
2). Signal transduction and cell apoptosis

PART II. Background: Pattern Genetics and Non-linear Cell Dynamics

A) Genomic organization of genes
B) Genomic regulation of genes expression pattern
1). Gene expression cascade
2). Transcription, translation and degradation
3). Clock Regulation of gene expression
4). Gene expression profiles
C) Signal transduction and controling of cytogenesis
1). Signaling Transduction Network
1.1). Receptors:
ion channel linked receptors enzyme-linked receptors: single pass a). intrinsic enzyme activities, b). coupled to intracellular enzymes
G-protein coupled: 7-pass serpentine a). activation of adenylate cylcase eg , glucagon receptor b). activation of PLC, angiotensin, vasopressin, bradykinin c). activation of phosphodiesterase: transducin in photoreception, cytoplasmic and nuclear receptors.
1.2). Cytoplasmic transducers: S/T kinases: PKA, PKC, MAPK, ERK, Raf, etc. phospholipases: PLC lipid kinases: PI3K, PKB/AKT a).
inhibition of caspase-8, raf, Rb phosphorylation and FKHRL1 b). activation of P21, E2F, NFkB and nitric oxide.
1.3). Nuclear targets
2). Transcription factors and signal pathways
3). Ontogeny of pituitary cell lineages differentiation
4). GH, LH secretion and gene regulated expression
D). Cell cycle and cell fate determination
1). Cell dynamics, cycle regulation
2). Cell mitotic, differentiation, prolification and apoptosis
3). Cell morphogenesis
4). Cell mutagenesis and evolution (genetic mutation)
E). Vascular, Neuro-Endocrine and Morphogenesis Pattern
1). Signalling dynamics and pattern formation
2). Cell lineage mapping and organogenesis pattern
3). Oscillation, morphogenesis and psychosomatic medicine
F). Genomic stratified (mutation) construction
1). Replication and homologous recombination
2). Multify stratum construction in genome
G). Structurity Theory of Pattern Genetics
1). Structure integration
2). Function adaptation
3). Construct stratification

PART III. Materials and Methods: Siganl Transduction and Genes Expression Patterns

A) Reverse genetics and genetic controlling
1). Efficient mobilization of transposon
Heritable insertions of the transposon can be generated in the germ line Genes that have been mutated by insertion can be rapidly identified using PCR reaction These insertions can subsequently be remobilized to generate deletion Knock out, and frame shift mutations by imperfect excision.
2). The Cre/loxP recombination system
Cre is a 38 kDa recombinase protein from bacteriophage P1 which mediates intramolecular (excisive or inversional) and
intermolecular (integrative) site specific recombination between loxP sites, which consists of two 13 bp inverted repeats separated by an 8 bp asymmetric spacer region.
3). The flippase/FRT system
The yeast flp recombinase mediates sitespecific recombination between cis-acting FRTs. When the FRTs are arranged as direct repeats flanking a segment of DNA, recombination leads to the excision of the intervening DNA, leaving behind a single FRT.
4). RNA interference
RNAi is a reverse genetic tool to induce posttranscriptional gene silencing (PTGS). The RNAi method has been attempted in a wide variety of organisms and shown to work with varying degrees of success in C. elegans, Drosophila, plants, Planaria, Trypanosome, Zebrafish, Xenopus, mouse embryo and mammalian cultured cells. The dsRNA can expression in cells for knock down gene expression in vitro and in vivo by stem cell injection.
B). Molecular cloning and identification of gene
1). Cell lines and tissue culture
2). EIA analysis
3). Molecular cloning and sequence
4). RT-PCR semi-quantitation
5). Sothern, northern blotting
6). Western blotting, pulse-chase experiment
7). cDNA microarrays
8). Reporter gene assay

PART IV Results and Discussion: Transcription Factors Regulatory Network

A). Signal Transduction Pathways Involved in GH, LHbeta Expression
1). Signal pathways of Egr-1, Sp1 mediate GH, LHbeta expression in response to GHRH, GnRH
2). GHRP-6 stimulate GH expression and Pit-1, c-Jun pathways
B). GnRH receptor pathways regulated involves steriod synthesis
C). Siganl transduction network involved in somatotroph, gonadotroph differentiation

PART V. Further: Artificial Biosystems and System Biological Engineering

A) Pharmaceutical genomics and drug discovery
1). Gene Expression cDNA profiles, PCR Shag, EST micro-array, proteomics
2). TCM Channel - Pharmacy, chemical genomics, pharmacognacy screen
B) Transgenics and bioreactor
1). Mammalian gland bioreactor
2). Avian oviduct bioreactor
3). Herb metabolism bioreactor
C) System biotechnology and bio-computation
1). Computation of molecules interaction
2). Cell automatics and bio-nanotechnology
Figures:
1. Cell Cycle and Fate Determination.
2. Automatic Cell and Bionic Computer.

(All rights reserved SysBioEng, please cite as:
B.J. Zeng, The Pattern Genetics - Non-linear Cell Dynamics, 2002 EU, Modified by Structurity Bulletin 2003)

  SysBioEng Home